LOCUS       sequence-124763-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 activity reporter (control) with mCherry and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    132..249
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        324..572
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     324..344
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     495..514
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(734..757)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
       61 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
      121 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      181 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      241 caaaattttc gggtttatta cagggacagc agagatccag tttggttagt accgggccct
      301 acgcgtccaa ggtcgggcag gaagagggcc tatttcccat gattccttca tatttgcata
      361 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
      421 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      481 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      541 ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacac gtttaagagc
      601 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt
      661 ggcaccgagt cggtgctttt tttggatcca attctaccgg gtaggggagg cgcttttccc
      721 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
      781 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
      841 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc c
//
