LOCUS       sequence-124771-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-T5
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(30..61)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 agggcggggc gtaaacgcca tgggcatgta gtcaaaagcc tccggtcgga ggcttttgac
       61 ttggctgagg agtgccgtta attaagttta cagacccagg gtacggaacg cgtcacgcag
      121 gtcgtcaact tcgtacagcg gcagcaggat acgacgcagc tgacgagaca gacgaacaga
      181 gttttcgtgc agaccttccc acgctttgaa ggtacggccg tggttttcaa cgaaggtgtt
      241 ccagcagtag aagtagtctt cgaaggtcat gatcgcgatc tgaacacccg cacggtgcag
      301 acgacgcaga ccttccggtt ccgctttacg gtcttcgcag aagtacagac gcgcggtgaa
      361 gatacgcaga gacaggttcg ggttaccacg caggaagtcc gcaaccagac gcgcgcagtc
      421 agagcacgga gaccaagaga tgaaccaggt aacacggtag caacgacccg ggtccaggtc
      481 ccagtcagag atgtaacgca ggaacagcag ttcaacgtgg caaccgtttt tgttacgcag
      541 gtaaccgaag tcacgagaga aagaggtcgc agagtcgcaa cgtttaacaa cgtagcacag
      601 gtaggtttca cgacgacctt tcgcccaacg aacgtttttg aactggtaca ggaattcgct
      661 acggttcatc agcagagagt ccatcttcag attccggttt acttaatctc gacttataac
      721 attttaattt tattttctcc attactgtct tgaattacta aagcccaagg tttatattct
      781 ggagcatctg aagttaatag cattacatt
//
