LOCUS       sequence-124772-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-U
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      26..57
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(84..710)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
ORIGIN
        1 cggggcgtaa acgccatggg catgtagtca aaagcctccg accggaggct tttgacttgg
       61 ctgaggagtg ccgttaatta agtttaaacc gccggagatt tggtggtgtg caggatttta
      121 acctggatca tgatagacag ttcagaacga cgtttttccg cacgtttcag ggttttttcc
      181 agccaacggt tttcgttcag ctggttgtga gaagactgga tgaagatttt acggcagcac
      241 tggtagtgtt cagaaaccat aacgttcaga ccaacaccgt tgtcacgcag gttccacaga
      301 ccgatctggt tacgcgcgtt tttttcgtag tacagtttgc acgcccagat tttcagggtg
      361 tgaccgttac cacgcagttc ctggttgtac cattccagga ttttttccgc gcagtccgcg
      421 cacggagacc aagaagagta ccagttgatg gtgaactgac ccgggttgtc acgcaggtat
      481 tcttcaactt tacggataga gaagatttcc gcgtggatac cacgttcggt accagactgc
      541 ggtttgttaa ccgcgtaacc ccagaagcac gcacgacgtt caccacgacg tttcagttcg
      601 aacagaacgt agcaacggtg agaaacagat tttttgttgt tgaagaactg ttttttgaag
      661 gtgtagatgt ccagtttttc gtggatacga acgtattccg cgtcggtcat cttcagattc
      721 cggtttactt aatctcgact tataacattt taattttatt ttctccatta ctgtcttgaa
      781 ttactaaagc ccaaggttta tattctggag catctgaagt taatagcatt acatt
//
