LOCUS       sequence-124775-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 activity reporter with mCherry and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    124..241
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        316..564
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     316..336
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     487..506
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(728..751)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
       61 ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca
      121 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      181 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      241 tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgggcc ctacgcgtcc
      301 aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac
      361 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
      421 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      481 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      541 atcttgtgga aaggacgaaa caccgagcac gcccccgtcc tcgtgtttaa gagctatgct
      601 ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
      661 gagtcggtgc tttttttgga tccaattcta ccgggtaggg gaggcgcttt tcccaaggca
      721 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
      781 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
      841 cttcgcgcca ccttctactc ctcccctagt caggaagt
//
