LOCUS       sequence-124776-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR gRNA expression vector with an improved l scaffold and 
            puro/BFP markers without WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    120..237
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        312..560
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     312..332
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     483..502
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(722..745)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
       61 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
      121 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
      181 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      241 gtttattaca gggacagcag agatccagtt tggttagtac cgggccctac gcgtccaagg
      301 tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      361 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
      421 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      481 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      541 tgtggaaagg acgaaacacc gggtcttcga gaagacacgt ttaagagcta tgctggaaac
      601 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      661 gtgctttttt tggatccaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
      721 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
      781 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
      841 gccaccttct actcctcccc tagtc
//
