LOCUS       sequence-124777-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR gRNA expression vector with an improved l scaffold and 
            puro/BFP markers without WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..86
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
       61 ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaactgcta gagattttcc
      121 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
      181 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
      241 agccagagag ctcccaggct cagatctggt ctaaccagag agactctggt ctaaccagag
      301 agacccagta caggcaaaaa gcagatcttg acttctttgg gagtgaatta gcccttccag
      361 tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct
      421 taaaggtacc tagcggccgc tcaattaagc ttgtgcccca gtttgctagg gaggtcgcag
      481 tatctggcca ctgccacctc gtgctgctcg acgtaggtct cgttgttggc ctccttgatt
      541 ctttccagtc tgtagtccac atagtagacg ccaggcatct tgaggttctt agcgggtttc
      601 ttggatctat atgtggtctt gatgtttgcg atcagatggc tcccgcccac gagcttcagg
      661 gccatgtcgt ttctgccttc caggccgccg tcagcggggt acagcgtctc ggtgaaggcc
      721 tcccagccga gtgttttctt ctgcatcaca gggccgttgg atgtgaagtt cacccctctg
      781 atcttgacgt tgtagatgag gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc
      841 acgcccccgt cctcgtatgt ggtgactctc tcccatgtga agccctcagg gaaggactgc
      901 ttgaagaagt cggggatgcc ctgggtg
//
