LOCUS       sequence-124780-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR gRNA expression vector with the conventional scaffold and 
            puro/BFP markers without WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    121..238
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..561
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     313..333
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     484..503
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        580..655
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(713..736)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
       61 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
      121 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      181 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      241 ggtttattac agggacagca gagatccagt ttggttagta ccgggcccta cgcgtccaag
      301 gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat acgatacaag
      361 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
      421 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      481 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      541 ttgtggaaag gacgaaacac cgggtcttcg agaagacacg ttttagagct agaaatagca
      601 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      661 ttggatccaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
      721 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
      781 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccac
//
