LOCUS       sequence-124781-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 activity reporter with GFP and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    121..238
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..561
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     313..333
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     484..503
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(725..748)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
       61 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
      121 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      181 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      241 ggtttattac agggacagca gagatccagt ttggttagta ccgggcccta cgcgtccaag
      301 gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat acgatacaag
      361 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
      421 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      481 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      541 ttgtggaaag gacgaaacac cgagcacgcc cccgtcctcg tgtttaagag ctatgctgga
      601 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      661 tcggtgcttt ttttggatcc aattctaccg ggtaggggag gcgcttttcc caaggcagtc
      721 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
      781 tcgcacacat tcc
//
