LOCUS       sequence-124783-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 activity reporter with GFP and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..775)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             complement(776..829)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(890..911)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
       61 gagcggccgc tcaattaagc ttgtgcccca gtttgctagg gaggtcgcag tatctggcca
      121 ctgccacctc gtgctgctcg acgtaggtct cgttgttggc ctccttgatt ctttccagtc
      181 tgtagtccac atagtagacg ccaggcatct tgaggttctt agcgggtttc ttggatctat
      241 atgtggtctt gatgtttgcg atcagatggc tcccgcccac gagcttcagg gccatgtcgt
      301 ttctgccttc caggccgccg tcagcggggt acagggtctc ggtgaaggcc tcccagccga
      361 gtgttttctt ctgcatcaca gggccgttgg atgtgaagtt cacgcccctg atcttgacgt
      421 tgtagatgag gcacccgtcc tggaggctgg tgtcctgggt ggcggtcagc acgcccccgt
      481 cctcgtaggt ggtgaccctc tcccatgtga agccctcggg gaaggactgc ttgaagaagt
      541 cggggatccc ctgggtgtgg ttgatgaagg tcttgctgcc gtagaggaag ctagtagcca
      601 ggatgtcgaa ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg
      661 tgccctcgta gggcttgcct tcgccctcgg atgtgcactt gaagtgatgg ttgtccacgg
      721 tgccctccat gtacagcttc atgtgcatgt tctccttaat cagctcgctg cccattgggc
      781 caggattctc ctccacgtca ccgcatgtta ggagacttcc tctgccctct cctccggacc
      841 cgccgccctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      901 gcaggaccat gtgatcgcgc ttctcgttgg gg
//
