LOCUS       sequence-124784-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9 fused with the Blasticidin 
            resistant gene at the C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-EF1a-Cas9Bsd-W
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..104
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             111..131
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catttcaggt gtcgtgggaa gcttggcgcg ccaccatgga ctataaggac cacgacggag
       61 actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga
      121 agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg
      181 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
      241 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
      301 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
      361 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
      421 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
      481 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
      541 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
      601 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
      661 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
      721 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
      781 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
      841 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctga
//
