LOCUS       sequence-124785-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9 fused with the Blasticidin 
            resistant gene at the C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-EF1a-Cas9Bsd-W
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..443)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(444..497)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(516..563)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgagcgg ccgctcagcc ctcccacaca
       61 taaccagagg gcagcaattc acgaatccca actgccgtcg gctgtccatc actgtccttc
      121 actatggctt tgatcccagg atgcagatcg agaagcacct gtcggcaccg tccgcagggg
      181 ctcaagatgc ccctgttctc atttccgatc gcgacgatac aagtcaggtt gccagctgcc
      241 gcagcagcag cagtgcccag caccacgagt tctgcacaag gtcccccagt aaaatgatat
      301 acattgacac cagtgaagat gcggccgtcg ctagagagag ctgcgctggc gacgctgtag
      361 tcttcagaga tggggatgct gttgattgta gccgttgctc tttcaatgag ggtggattct
      421 tcttgagaca aaggcttggc cattgggcca ggattctcct ccacgtcacc gcatgttagg
      481 agacttcctc tgccctctcc tccggacccg ccgccctttt tcttttttgc ctggccggcc
      541 tttttcgtgg ccgccggcct tttgtcgcct cccagctgag acaggtcgat ccgtgtctcg
      601 tacaggccgg tgatgctctg gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg
      661 tacctcttcc ggtcgatggt ggtgtcaaag tacttgaagg cggcaggggc tcccagattg
      721 gtcagggtaa acaggtggat gatattctcg gcctgctctc tgatgggctt atcccggtgc
      781 ttgttgtagg cggacagcac tttgtccaga ttagcgtcgg ccaggatcac tctcttggag
      841 aactcgctga tctgctcgat gatctcgtcc aggtagtgct tgtgctgttc cacaaa
//
