LOCUS       sequence-124788-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-U3
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      44..75
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(102..728)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
ORIGIN
        1 ctgcgatgag tggcagggcg gggcgtaaac gccatgggca tgtagtcaaa agcctccgac
       61 cggaggcttt tgacttggct gaggagtgcc gttaattaag tttaaaccgc cggagatttg
      121 gtggtgtgca ggattttaac ctggatcatg atagacagtt cagaacgacg tttttccgca
      181 cgtttcaggg ttttttccag ccaacggttt tcgttcagct ggttgtgaga agactggatg
      241 aagattttac ggcagcactg gtagtgttca gaaaccataa cgttcagacc aacaccgttg
      301 tcacgcaggt tccacagacc gatctggtta cgcgcgtttt tttcgtagta cagtttgcac
      361 gcccagattt tcagggtgtg accgttacca cgcagttcct ggttgtacca ttccaggatt
      421 ttttccgcgc agtccgcgca cggagaccaa gaagagtacc agttgatggt gaactgaccc
      481 gggttgtcac gcaggtattc ttcaacttta cggatagaga agatttccgc gtggatacca
      541 cgttcggtac cagactgcgg tttgttaacc gcgtaacccc agaagcacgc acgacgttca
      601 ccacgacgtt tcagttcgaa cagaacgtag caacggtgag aaacagattt tttgttgttg
      661 aagaactgtt ttttgaaggt gtagatgtcc agtttttcgt ggatacgaac gtattccgcg
      721 tcggtcatct tcagattccg gtttacttaa tctcgactta taacatttta attttatttt
      781 ctccattact gtcttgaatt actaaagccc aaggtttata ttctggagca tctgaagtta
      841 atagcattac at
//
