LOCUS       sequence-124789-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-U4
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      37..68
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(95..721)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
ORIGIN
        1 gagtggcagg gcggggcgta aacgccatgg gcatgtagtc aaaagcctcc gaccggaggc
       61 ttttgacttg gctgaggagt gccgttaatt aagtttaaac cgccggagat ttggtggtgt
      121 gcaggatttt aacctggatc atgatagaca gttcagaacg acgtttttcc gcacgtttca
      181 gggttttttc cagccaacgg ttttcgttca gctggttgtg agaagactgg atgaagattt
      241 tacggcagca ctggtagtgt tcagaaacca taacgttcag accaacaccg ttgtcacgca
      301 ggttccacag accgatctgg ttacgcgcgt ttttttcgta gtacagtttg cacgcccaga
      361 ttttcagggt gtgaccgtta ccacgcagtt cctggttgta ccattccagg attttttccg
      421 cgcagtccgc gcacggagac caagaagagt accagttgat ggtgaactga cccgggttgt
      481 cacgcaggta ttcttcaact ttacggatag agaagatttc cgcgtggata ccacgttcgg
      541 taccagactg cggtttgtta accgcgtaac cccagaagca cgcacgacgt tcaccacgac
      601 gtttcagttc gaacagaacg tagcaacggt gagaaacaga ttttttgttg ttgaagaact
      661 gttttttgaa ggtgtagatg tccagttttt cgtggatacg aacgtattcc gcgtcggtca
      721 tcttcagatc ctcctttact taatctcgac ttataacatt ttaattttat t
//
