LOCUS       sequence-12479-e       15699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15699)
  TITLE     Stainier unpublished
REFERENCE   2  (bases 1 to 15699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: bActin-RSG-BiSKi
FEATURES             Location/Qualifiers
     source          1..15699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(86..119)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             143..820
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(273..291)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(323..343)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     541..560
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     735..758
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     intron          905..970
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1100..1120
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1545..1679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1734..1767)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1810..1819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1816..1818,1819..1821,1822..2535)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1816..1818,1819..1821,1822..2532)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1861..1882)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2122..2141)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2469..2490
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2659..2740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2812..2830)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(2894..2912)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2951..2967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2951..2967)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2964..2986)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2975..2991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2999..3005,3006..3023,3024..3029))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3044..3065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3182..3199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3353..3941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3433..3452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4112..4903,4904..4972))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4735..4754
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4973..5077)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5104..5559
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5191..5210)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5401..5422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5685..5707
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5699..5716
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5700..5716
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5744..5763
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5744..5762
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5788..5804
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5869..5887
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtgggct gcaggaattc gatatcaagc ttatcgatac cgtcgactct agaggatcag
       61 cttgggctgc aggtcgaggg acctaataac ttcgtatagc atacattata cgaagttata
      121 ttaagggttc cggatcctcg agatggcctc ctccgaggac gtcatcaagg agttcatgcg
      181 cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
      241 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
      301 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt
      361 gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg
      421 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
      481 gcaggacggc tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg
      541 ccccgtaatg cagaagaaga ctatgggctg ggagccctcc accgagcgcc tgtacccccg
      601 cgacggcgtg ctgaagggcg agatccacaa ggccctgaag ctgaaggacg gcggccacta
      661 cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta
      721 ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca
      781 gtacgagcgg gccgagggcc gccaccacct gttcctgtag cctcgaggga tctttgtgaa
      841 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
      901 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
      961 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     1021 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     1081 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     1141 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     1201 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     1261 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     1321 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     1381 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     1441 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     1501 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     1561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     1681 gacatggtaa gtaagcttat ccccggagct tgggctgcag gtcgagggac ctaataactt
     1741 cgtatagcat acattatacg aagttatatt aagggttccg gatcggatcc cctgacggat
     1801 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1861 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1921 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1981 gtgccctggc ccaccctggt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2041 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2101 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2161 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2221 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     2281 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     2341 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2401 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     2461 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2521 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     2581 agggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2641 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2701 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2761 aaactcatca atgtatctaa tcgagggggg gccggtaccc agcttttgtt ccctttagtg
     2821 agggttaatt gcgcgctagg gataaactag ttctagagcg gccgccaccg cggtggagct
     2881 ccagcttttg ttccctttag tgagggttaa ttgcgcgcta gggataacag ggtaatttgg
     2941 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3001 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3061 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3121 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3181 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3241 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3301 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3361 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3421 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3481 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3541 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3601 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3661 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3721 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3781 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3841 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3901 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3961 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4021 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4081 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4141 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4201 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4261 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4321 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4381 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4441 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4501 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4561 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4621 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4681 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4741 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4801 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4861 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4921 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4981 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5041 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5101 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5161 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5221 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5281 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5341 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5401 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5461 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5521 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
     5581 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     5641 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     5701 taaaacgacg gccagtgata gggataacag ggtaatgcgc gcgtaatacg actcactata
     5761 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
     5821 attcctgcag cccgggggat ccactagcta gttctagagc ggccgcggaa ttaaccctca
     5881 ctaaagggaa cgaattcgga tctcagagct tctcataagc aaaacacaag ctgagtgtgc
     5941 aatgaatgag atgcttgctt tgatgactaa gtttggcaat cgctacctgc tagtcctttc
     6001 cttatttgga gcctgagaag ctggagataa agctaagctc cgcccatcca ctcgcatact
     6061 ggtaattgtt tggtttctaa acagtgttag atcgggcgga gcatgagagt gatggtgtga
     6121 gtgagtaaga acgtgtgcgt gtttgggaga ggcagattaa gagtgagaga gtttctgaaa
     6181 gggaacgaca gactgttgat agtttatctg ggcttattga ctgtgtcaag cgttagtgtg
     6241 tgaaagtgca aagcgagcaa atcaatgtga acaggacatt gaggaaaaac agtgatatga
     6301 aatcagcttt ctgaagttga gacatcacca gtcatcattg ccttgccaaa ggctgactat
     6361 atatggatat gacctcgctg tcattatttc aaaccacttc cttcttactc atcgtgaggc
     6421 agatcatagg caacccttca aatggaggaa tgaatgagaa acactgttgc tttggcagaa
     6481 agacacaaca aatgaaaatg ctacaaacaa acaaggaaca ttactgtatg atgtaaaagc
     6541 tgaatatata tggattttac atacgaatat gtgtcaatga aagagtctca cagtgtgcag
     6601 agtgaaacta cagcaagcca gaaataaaat aaatgtcatt gtttgatcaa aactccccag
     6661 cttctctaac ttagcgtttc ttcattcgtt aggcctagaa gtttcaatct gatttccatg
     6721 agaaagtccc atgtgacaag ggccttgaat tcttgacatt gttaccatgt gacacactta
     6781 aagatttcct tttatggcgg ttgctgttac atcgaccaga gggcgaggcc taacacaacc
     6841 aaagtgcaca gaacggtcta cagctaaagc ccttgaggaa ataaatatga agaatccttt
     6901 tgtggacaaa gtttgaggaa aaatgcagcg catgcctagc aggatgtctt gttatgccgt
     6961 atctcatatc tttgcatcga aattcttcac atttctaaag atgtcaagcg ttgctatcat
     7021 acttacatgt catggagttt tatagtactg gaggtatatt ataatattga caattttaca
     7081 atgcttttca tatatttttt atatttaaga tgttatattt ctctaaatca tatgcacatt
     7141 atgtgcaagt cattttccag agttttgtgc cacacctaag ccaaaaagct tctgaattgc
     7201 tcaacaagaa agaacatgtc tgttgcctct tagtatagcc aatgcatcat tatagaaagg
     7261 gcaaatgact tgtaaggatt gtggcaaaga gttatagtca tcagaaaacc tcagcagggt
     7321 tttcctttag attactcata acaaaaaaca aaacacaaat gctatattga gcccaaaatt
     7381 aagcagttat ttgtttttta gcacagcatt atattaagga ttaactattc ataaaacaac
     7441 tgaacaaata aaaagccctt taatgtaaga attacattaa ttatagaaag cagacaacat
     7501 aattaaactg aaagttttta taaactctat gctagattct gctggagcta attaagcgcc
     7561 cagatgaggc aaacagctgt ttacgaagtc atttaaaatc ttgtttgcat gctgtactgt
     7621 tgtttctttc cacacataag tgagagtgag gtgtgtgtgc actgcacagc atcacgataa
     7681 aaggagtaag cactactact aagttgtaca ttccttagtt ggctaagctt tctcccacag
     7741 cagaatattt caatgcgatt tcctgtgcaa acttgctgaa gaaatgacat cctttaaaaa
     7801 cgcacttcct gtaagagatg cataggtcat tcccttattt ggcaactaac accggccttt
     7861 ggttttcagg gagtggaatt tacatcgttt aaggaaagag ctttatttca atcaacatat
     7921 ttactttacc agatcgaata tgttgaaact ccaagcttaa aaagacttaa ttattagatc
     7981 aatgccaaac attcagcaag actggcagtg ctattaaagg tgggctgaaa gttgatgtat
     8041 tcacttcaac acaaatgacc ctaatatgaa cctctgccaa tattaatgtt ggacctgggc
     8101 cttgggatac ctcagtctag ccagagatgc tgtgagcgcc acacctcatc agtttcacac
     8161 agacatcact caggaatgac agatatttca tgcgcaagag acattaacat gaaactcttg
     8221 agcaacaaag ctctaatgaa taaggtatta acggcaaaca aaatgaatgg cggcaatctt
     8281 ttgttattta cattacggtt tcggtggagc caatttgttt tgcaaatgtc taacatgatt
     8341 atttaaagtg actataagca cacattcttg aaggcaatat ctatatcccc cttaaagatt
     8401 gcatatttgt ctttcagaaa tgaaaaagcc cctaattcat ttatttaacg gtcatttgtt
     8461 cattttattt acagcttagt ccgtttattt atcaggagtt gccacagcgg aatgaaccac
     8521 caacttatct agcatgtttt atgcagcaga tgctcttcca gccgcaaccc atccctcgaa
     8581 aacatccata cactctcatt cacacacaca ctacggacaa tttaacttac ccaattaacg
     8641 gtcatttaaa ataaggctaa agacaacacc acggtgagta aataatgaca gtgtttattg
     8701 ttgggtaaac tatgccttta aaagaggatc atttccgtct tgcactacat attttgaatg
     8761 ttaaaactgc tcttgaacac ataaaaatct attatgctaa agtatctgct tgaattactc
     8821 agcttcctgt ggcaggccca agctatggtg cttggcacag taatctctcc catgctggtt
     8881 taaaggctga ctgagtctga agtgtggacg agcacgtctg actcattcca gggccaatcc
     8941 cctgctcagc cttctctctc tctcacgggc agacagatgg tggaggctag attaggaggc
     9001 ctagggtctt ggtacaaaag cagatattgc tagaaactga cccaaacaca gtggtcccaa
     9061 atcataaccc ccttcagctc tgggtaaatc cccatgcatt tggaggagat aactgcaggc
     9121 cacatagtgg tggattcaat gtgtagcatg cattaaatgt tataatcagt gtcaaatgtt
     9181 caaaactatg caatcgtaac gcttggagtt gacaaattat gaatatgatt gatgcaatct
     9241 ctagagcagc atgttgaaat gatcccaaca tgacttattt atatctcagt agcagagatt
     9301 gagcccctgt ttagcagtgg gcacagtggg gtgctaataa gcccttgatc tctattgatc
     9361 agccagcact gaagcgagca gaactttctt atacagagag cattatgtac acattttgaa
     9421 agcatatgca gccttaaaga gatagttcac cccaaaatgt acaagttttc cactttgtca
     9481 ctccaaacct tggtgactgt cttctgtgaa acacaaaaca aaaatatttt ttgactgttt
     9541 ttgtcagtag aataagtgtt tagaaataat cattcaatca ttcattcatt ttctttggct
     9601 taatcccttt attcatcaaa agtagccaca gcggaatgaa ccgccaactt atccagcata
     9661 tggatgccct cccagctgca acctaatcat tcaatgtaat aacaaaacgt taatcttcgc
     9721 aggaactacc gctaaaatat gacaagtgac aaacccaagt atgcaaagca atatgttgac
     9781 aattttgtac atgcaagcaa acagccataa atggactagc ggtcctcccc taccagcttt
     9841 tacagcaaag aggaagtgaa gctttgcaat ggcaagaaag gacacggtga caactgttct
     9901 gctgaggaac ataaatgagg aacaaggctt ttttttcttg attagtatca aatcctgttg
     9961 caagtggttt gacaatacaa atgaagacac aaaatatgtc tttgtctgct gaagtaacac
    10021 cattttgaat gtaaacccac tatgatgtat cttaaataat gatttgtcac ataatattgc
    10081 ataagagaaa agtatctaat tatttacact catataaggc gatgagtaac cacatatgaa
    10141 tccatttggg tcataatgta gggggttaat ccaatctcta gactaagagg ccagctgtca
    10201 gtatatatgt gacttccttt caagcctgag tatctgcacc atcaagcaac caagaacaag
    10261 tcaatctaaa atcaatcggt ggtccttctg ctgtttatag gacaaatgaa gggcttcgct
    10321 tccgctattc ccaagacaag gagggtaaaa aaaaaataca taaaagaaaa taaggaaaga
    10381 aaaaatatca ggaattccag tttgaagaaa cttttcaaga ctcacctttt cactgttggt
    10441 cttaatttag gttgattatt tttgtcaatt taataattac attgtattgt tcatatttta
    10501 ttgtcttatt gtggtgtttt tacagctttt ttattgttct gctttgttac tgtttgcctt
    10561 gtatgcattg tagcgctttg ggtctgagaa aagcgcacta taaataaact gtattattat
    10621 tattagggat ggacatcacg gtggcgcgat gggtggcaca attacctcac agcaagtagg
    10681 ttgctgttta gagccccggc tggatcagtt ggcagttctg tgtggagttt gcatgttctc
    10741 cctgttcaag taggtttcct cccggtgctc tggtttcccc tcacaagtcc aaagacatga
    10801 tgtataggtg aatggggtaa gctaaattga ccatagtgag tgtgtgtgtg tgtgtgaatg
    10861 agagtgtata ggtgtttccc agtgttaggt tgcaactgga aggacatctg ctgtgtaaaa
    10921 catatgcagg ataagttggc ggttcattcc cctatggtga cccctggtta attaaaggac
    10981 taagttgaaa atgaatgaat gaatgaatga ttagagataa accgatacca cttttttaca
    11041 aaccgatatg agtacaagta taatagtttc tgttactcga caatactgag taccgattct
    11101 gatacttctt ggatttggtt tcaatgtaat taatttgaaa gaaggtttta tttcttgcct
    11161 gtcgcaagga tgaacacaaa aatctttacc agaaggatat tttaagctaa gaatatacgc
    11221 acgttgttga caaccctgta tcgattaccg attaaacgtg gacatttaaa ggataagtta
    11281 tgcttgcgcc atcacaactt tacacattac atgatcaaag atcatattga atattcggtt
    11341 tattcgtcag aagcctatag atttgttaaa agtgtatttt ataaacggat ggtttataaa
    11401 gactaagcta ttaatatatc agacattcat taatacatca agacatcgat gtcaggtagg
    11461 acaacaaatc acatccatgt aatgaaatct tgttgagtgg tattggtgcg tggtttcagc
    11521 atttcatgtg caatacgagt aagaatattt taaccaagta tcgacccaaa acctgatact
    11581 ggtatcggtg catcccttat tattattatt atcaaaaaca cacttgtttt atttatgtgg
    11641 gtgccaaaca acaaactatg aaatgcagca tgacacgccc aaaaagtgtt cctttgagaa
    11701 caacttcata aatatgtttc ttatcattat gcatgaatgg tgtactggga gagctttggt
    11761 atcagaattg agataagaga ctttggcatt ctaaaggaca atgacattat cacactgaca
    11821 gtcaacaaca ctttattgct ctggaaatct tatgtcatgg ggcaaagctg aggttcatta
    11881 ggacagtacc ttatttagag atgaatggga aggctgtagt gggctgctat cagggaaggg
    11941 tggtggtcct cgggggaact atggactcac aaaagcacat ctgctgtaac aagatcccct
    12001 gtgcaatcta caaaagttac aaagagaagc atggttggtt aaaaaataga attgactgga
    12061 tatgctgaat gcattgtttg ttgtgtgtta catcacatta accacagatt ggtgctaaat
    12121 atttgcttat cttataaaaa cctgtgtgtt tataaagggg ggtgtggatg gatgcttggc
    12181 attctttgaa aagatgttgg gggatggaag gaggagacct tattgatgag tatccagttt
    12241 gcagataaca agtttttaga cattcatact atgatgctgt ttatgtctgt tttttctcat
    12301 tttttctata cacaaacagc tataaaacat tcacttttat atctgatgct gtatattaaa
    12361 tttcgttgta tttattctgt ttgctctgtt gggtcatagc tagggccaga cagaatctgc
    12421 tgacattttt tgctttttct gcagagaatt ttgtaaacaa tctgtgattt atgcgagatt
    12481 ttttcgggag tatcgtaact aaaaacttac tatatgaaat aaaaaaaaat aatacctttt
    12541 taacttttaa taaatatata caatgcaagt ccacctagat ccaattattt ggtcgacaaa
    12601 gcaagtctct cacataatat atcttctaaa agacagaaaa cattactttc agaccatatt
    12661 gtaatcaaat gaacactttc atattagtca ataatattac ggaaatgtat ttaaaaactg
    12721 aataaacata tatttacacc tttgtcttta tttctagagt gcttttacaa tgtagattgt
    12781 gtcaaagcag cttaacttag aggattagta tagtaaattg aaactgtgtc agtccatttt
    12841 ttaagagttt aagttcagtt cagtttagtt cagtgtgagt taatttttac tgctgagagt
    12901 ccaaacacta aagagcaaat ccaaatacac aagtcaataa ataaactcaa tgatgggcta
    12961 aagatctggg gaaatctgca gatttctgtg cgcacagatt ctgtgtgggc ctagtcatta
    13021 cctatactca acgtttactc tgagaaactt ccatatttta gaggtaaaat gctcagaatt
    13081 gtaaaaagtc ttgtcaaaat acttggaatt tgccaaaaac aaaataaaag tcacatcacc
    13141 tcattgtcct tggtatcagc aagcactttt aacacaatca gctgtttttg tttataactc
    13201 ccactgaagg ccatgtgcat tactcccaag ttacacagta aaaagtcaca tgtagtttat
    13261 catgatcact gctgctcaag cacagcttta tctgttattc agtgtggcaa tatctgaaat
    13321 gtgtgcaaaa tattatttat tgtagctgat caccaacact ttcagtgttt tttttttcta
    13381 cttttaatga gaattatgtt cttaggattt atccttataa acctctttaa aaagaaatgt
    13441 tatgtctgca tagacacgac atgaaattat gaacagaaaa tttaactatt taagatcaag
    13501 acttgacata taacatttct aatgaacatt aaacctgagg taggcccact tgcaaaaatg
    13561 taactgctat cactcacttt atacatcatt gtgtaacttg tacacacaaa aatagtgaag
    13621 caatgtggca tggaatgcag gtcccattca ttcaaagtta ttaaagcact ttcttaaagt
    13681 gcattactac tgagctaaga ccagctagtt gctcatacaa catactacaa gtagtgtcat
    13741 cctaatttgg ataaaaaaaa tgtccatttt atacatggaa aagtactcat tagatccccc
    13801 attgtttgta ttacggtatt tcgtgaacac aagaggtaaa tgacctacag agctgctgtt
    13861 gtgttagatt gaaaacacaa cacaggatca tggaggcatc catcattatg acgcattctg
    13921 ccacttgtaa ctctgcacag actttttgaa aagtttaatt gagtcttggc gttgtcgcag
    13981 aaaaatacga aaaattctgt gttaaaattt gcaaataaaa attccagttt tataggaaat
    14041 cgccatggcc ttgttcttta accagttaag cttccccttc tttcactctc aagttgcaag
    14101 aagcaagtgt agcaatgtgc acgcgacagc cgggtgtgtg acgctggacc aatcagagca
    14161 cagagctccg aaagtttacc ttttatggct agagccgggt atgtgccgtc atataaaaga
    14221 gcgcgcccag cttttcagcc tcactttgag ctcctccaca cgcagctagt gcggaatatc
    14281 atctgcttgt aacccattct cttaagtcga caacccccca aacccaaggt gagttggttt
    14341 tacagttttt aagcgtttaa ttatagtttc tatctattaa tgttaagtaa aatgtgtaaa
    14401 tggataaata tgatttgatg aagtctttag acagttcata acggtctctt tgtagttatc
    14461 tactaggttt gatttataag tggtggttct tttgataatc tatgttacat ttaataaata
    14521 ttggctgtat cttaactttt ttgtagcgtt agtggatgtg gcaggtgaga atgtcggtaa
    14581 tttaacgtga ccagtcgtag gcacgacatt gaacattgaa taggccggtg tgaaatgggt
    14641 gttaagtctt aatataaagt tgtgttctgg ctaatctctc tttaacagcc ataaatgtga
    14701 tttttaacta cagttttgag ggtgctggtg agcatgttgc acacttgatg gatagccggc
    14761 atgggaagtt ctttgtgcag gcagtgctgc agcagggtgt gacctacttt agctagccgg
    14821 ctaaccagct ctcatctgat gtaacgtaaa ccccatcaat ctaacatcgg ctcattcgtg
    14881 ctttataaga tcgaagatct tgttattagt aacgggtatt ggcatttagt tttaacttgg
    14941 ttaattttct ttttgttatg tgttctattt aaaatgtagc ctgcagcccc cctctgtctt
    15001 taatgaagct gaagattaac gttaacgtta gtgaagtgtt gtatccgcta tgaaagattt
    15061 gaattttaga ggtgtggtcc actttaataa cggctgcgga gttctctggc tgcacttcag
    15121 ccgctgtgac tgtcgaagca gagcccatca gtttttcagg atctcggtga tttttgaggc
    15181 tagactatga actgaaccga ctgagagtga ccgtccagaa atgctttagt gtgaagtctc
    15241 cgctcacgag gagtccgtgg actgactgca gatctgtgac gcagtaacac ccggcggcag
    15301 acacccgttg aaatgcggtt gtgtaattga tcgcaggcga gggtggaaga ggatgtgaaa
    15361 cttcatttgt gtaaaattta gggagtggtc ctggtgtgat gaatgtcgaa atctgttcct
    15421 ttttactgag ccctacgact ctggctgagt gccacaccgc cggcagccgc aaagcggctc
    15481 aaaccattgc cttttatggt aataatgaga gaatgcagag ggacttcctt tgtctggcat
    15541 atctgaggcg cgcgctgtca ctagcgccca ccagcggtca gactgtagaa tgcagagcaa
    15601 aacaggaagt tgactccaga tggtcacatg cttgctgaaa cgcctctccc tggcagcagt
    15661 gcaattctaa cagtttccct ttcttttaca gttcagcca
//
