LOCUS       sequence-124790-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-V
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      36..67
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(94..720)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
ORIGIN
        1 agtggcaggg cggggcgtaa acgccatggg catgtagtca aaagcctccg accggaggct
       61 tttgacttgg ctgaggagtg ccgttaatta agtttaaacc gccggagatt tggtggtgtg
      121 caggatttta acctggatca tgatagacag ttcagaacga cgtttttccg cacgtttcag
      181 ggttttttcc agccaacggt tttcgttcag ctggttgtga gaagactgga tgaagatttt
      241 acggcagcac tggtagtgtt cagaaaccat aacgttcaga ccaacaccgt tgtcacgcag
      301 gttccacaga ccgatctggt tacgcgcgtt tttttcgtag tacagtttgc acgcccagat
      361 tttcagggtg tgaccgttac cacgcagttc ctggttgtac cattccagga ttttttccgc
      421 gcagtccgcg cacggagacc aagaagagta ccagttgatg gtgaactgac ccgggttgtc
      481 acgcaggtat tcttcaactt tacggataga gaagatttcc gcgtggatac cacgttcggt
      541 accagactgc ggtttgttaa ccgcgtaacc ccagaagcac gcacgacgtt caccacgacg
      601 tttcagttcg aacagaacgt agcaacggtg agaaacagat tttttgttgt tgaagaactg
      661 ttttttgaag gtgtagatgt ccagtttttc gtggatacga acgtattccg cgtcggtcat
      721 cttcagattc cggtttactt aatctcgact tataacattt taattttatt ttctccatta
      781 ctgtcttgaa ttactaaagc ccaaggttta tattctggag catctgaagt taatagcatt
//
