LOCUS       sequence-124792-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-V3
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      17..48
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(75..701)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
ORIGIN
        1 aacgccatgg gcatgtagtc aaaagcctcc gaccggaggc ttttgacttg gctgaggagt
       61 gccgttaatt aagtttaaac cgccggagat ttggtggtgt gcaggatttt aacctggatc
      121 atgatagaca gttcagaacg acgtttttcc gcacgtttca gggttttttc cagccaacgg
      181 ttttcgttca gctggttgtg agaagactgg atgaagattt tacggcagca ctggtagtgt
      241 tcagaaacca taacgttcag accaacaccg ttgtcacgca ggttccacag accgatctgg
      301 ttacgcgcgt ttttttcgta gtacagtttg cacgcccaga ttttcagggt gtgaccgtta
      361 ccacgcagtt cctggttgta ccattccagg attttttccg cgcagtccgc gcacggagac
      421 caagaagagt accagttgat ggtgaactga cccgggttgt cacgcaggta ttcttcaact
      481 ttacggatag agaagatttc cgcgtggata ccacgttcgg taccagactg cggtttgtta
      541 accgcgtaac cccagaagca cgcacgacgt tcaccacgac gtttcagttc gaacagaacg
      601 tagcaacggt gagaaacaga ttttttgttg ttgaagaact gttttttgaa ggtgtagatg
      661 tccagttttt cgtggatacg aacgtattcc gcgtcggtca tcttcagatt ccggtttact
      721 taatctcgac ttataacatt ttaattttat tttctccatt actgtcttga attactaa
//
