LOCUS       sequence-124793-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-V5
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      39..70
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(97..723)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
ORIGIN
        1 atgagtggca gggcggggcg taaacgccat gggcatgtag tcaaaagcct ccgaccggag
       61 gcttttgact tggctgagga gtgccgttaa ttaagtttaa accgccggag atttggtggt
      121 gtgcaggatt ttaacctgga tcatgataga cagttcagaa cgacgttttt ccgcacgttt
      181 cagggttttt tccagccaac ggttttcgtt cagctggttg tgagaagact ggatgaagat
      241 tttacggcag cactggtagt gttcagaaac cataacgttc agaccaacac cgttgtcacg
      301 caggttccac agaccgatct ggttacgcgc gtttttttcg tagtacagtt tgcacgccca
      361 gattttcagg gtgtgaccgt taccacgcag ttcctggttg taccattcca ggattttttc
      421 cgcgcagtcc gcgcacggag accaagaaga gtaccagttg atggtgaact gacccgggtt
      481 gtcacgcagg tattcttcaa ctttacggat agagaagatt tccgcgtgga taccacgttc
      541 ggtaccagac tgcggtttgt taaccgcgta accccagaag cacgcacgac gttcaccacg
      601 acgtttcagt tcgaacagaa cgtagcaacg gtgagaaaca gattttttgt tgttgaagaa
      661 ctgttttttg aaggtgtaga tgtccagttt ttcgtggata cgaacgtatt ccgcgtcggt
      721 catcttcaga ttccggttta cttaatctcg acttataaca ttttaatttt attttctcca
      781 ttactgtctt gaattactaa
//
