LOCUS       sequence-124795-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For bacterial expression of recombinant human PRC1 tagged with His..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Nixon FM, Gutierrez-Caballero C, Hood FE, Booth DG, Prior IA, Royle 
            SJ
  TITLE     The mesh is a network of microtubule connectors that stabilizes 
            individual kinetochore fibers of the mitotic spindle.
  JOURNAL   Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635.
  PUBMED    26090906
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-06-19"; volume: "4. doi"; pages: " 10.7554/eLife.07635"
COMMENT     Sequence Label: His6-PRC1
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaaggagata tacatatgcg gggttctcat catcatcatc atcatggtat ggctagcatg
       61 aggagaagtg aggtgctggc ggaggagtcc atagtatgtc tgcagaaagc cctaaatcac
      121 cttcgggaaa tatgggagct aattgggatt ccagaggacc agcggttaca aagaactgag
      181 gtggtaaaga agcatatcaa ggaactcctg gatatgatga ttgctgaaga ggaaagcctg
      241 aaggaaagac tcatcaaaag catatccgtc tgtcagaaag agctgaacac tctgtgcagc
      301 gagttacatg ttgagccatt tcaggaagaa ggagagacaa ccatcttgca actagaaaaa
      361 gatttgcgca cccaagtgga attgatgcga aaacagaaaa aggagagaaa acaggaactg
      421 aagctacttc aagagcaaga tcaagaactg tgcgaaattc tttgtatgcc ccactatgat
      481 attgacagtg cctcagtgcc cagcttagaa gagctgaacc agttcaggca acatgtgaca
      541 actttgaggg aaacaaaggc ttctaggcgt gaggagtttg tcagtataaa gagacagatc
      601 atactgtgta tggaagaatt agaccacacc ccagacacaa gctttgaaag agatgtggtg
      661 tgtgaagacg aa
//
