LOCUS       sequence-124797-        9334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human FOXA1 with C-terminal V5 tag, puromycin 
            selection marker, 3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas 
            P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC, 
            Kantoff PW, Brown M, Loda M, Long HW, Freedman ML
  TITLE     The androgen receptor cistrome is extensively reprogrammed in human 
            prostate tumorigenesis.
  JOURNAL   Nat Genet. 2015 Nov;47(11):1346-51. doi: 10.1038/ng.3419. Epub 2015 
            Oct 12.
  PUBMED    26457646
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3419"; 
            journalName: "Nat Genet"; date: "2015-11"; volume: "47"; issue: 
            "11"; pages: "1346-51"
COMMENT     Sequence Label: pLX302_FOXA1-V5
FEATURES             Location/Qualifiers
     source          1..9334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    597..621
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2038..2062)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2064..2105
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2147..2735
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2200..2220)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2618..2629)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2807..3040
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3118..3239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3155..3174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3209..3228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3279..3414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3434..3453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3435..3453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3463..3480)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3463..3479)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3472..3494)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3621..4076
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3708..3727)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3918..3939
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4102..4206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4207..4275,4276..5067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4425..4444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5238..5826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5727..5746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5980..5997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6114..6135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6150..6155,6156..6173,6174..6180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6188..6204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6193..6215
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6212..6228
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6212..6228
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6247..6267
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6249..6267
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6295..6521
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6522..6702
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6749..6874
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7367..7600
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7785..7829
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8004..8504
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8423..8441
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8526..9125
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8526..8545)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9022..9042
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9167..9284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactgtcg ggatcaacaa
      601 gtttgtacaa aaaagttggc atgttaggaa ctgtgaagat ggaagggcat gaaaccagcg
      661 actggaacag ctactacgca gacacgcagg aggcctactc ctccgtcccg gtcagcaaca
      721 tgaactcagg cctgggctcc atgaactcca tgaacaccta catgaccatg aacaccatga
      781 ctacgagcgg caacatgacc ccggcgtcct tcaacatgtc ctatgccaac ccgggcctag
      841 gggccggcct gagtcccggc gcagtagccg gcatgccggg gggctcggcg ggcgccatga
      901 acagcatgac tgcggccggc gtgacggcca tgggtacggc gctgagcccg agcggcatgg
      961 gcgccatggg tgcgcagcag gcggcctcca tgaatggcct gggcccctac gcggccgcca
     1021 tgaacccgtg catgagcccc atggcgtacg cgccgtccaa cctgggccgc agccgcgcgg
     1081 gcggcggcgg cgacgccaag acgttcaagc gcagctaccc gcacgccaag ccgccctact
     1141 cgtacatctc gctcatcacc atggccatcc agcaggcgcc cagcaagatg ctcacgctga
     1201 gcgagatcta ccagtggatc atggacctct tcccctatta ccggcagaac cagcagcgct
     1261 ggcagaactc catccgccac tcgctgtcct tcaatgactg cttcgtcaag gtggcacgct
     1321 ccccggacaa gccgggcaag ggctcctact ggacgctgca cccggactcc ggcaacatgt
     1381 tcgagaacgg ctgctacttg cgccgccaga agcgcttcaa gtgcgagaag cagccggggg
     1441 ccggcggcgg gggcgggagc ggaagcgggg gcagcggcgc caagggcggc cctgagagcc
     1501 gcaaggaccc ctctggcgcc tctaacccca gcgccgactc gcccctccat cggggtgtgc
     1561 acgggaagac cggccagcta gagggcgcgc cggcccccgg gcccgccgcc agcccccaga
     1621 ctctggacca cagtggggcg acggcgacag ggggcgcctc ggagttgaag actccagcct
     1681 cctcaactgc gccccccata agctccgggc ccggggcgct ggcctctgtg cccgcctctc
     1741 acccggcaca cggcttggca ccccacgagt cccagctgca cctgaaaggg gacccccact
     1801 actccttcaa ccacccgttc tccatcaaca acctcatgtc ctcctcggag cagcagcata
     1861 agctggactt caaggcatac gaacaggcac tgcaatactc gccttacggc tctacgttgc
     1921 ccgccagcct gcctctaggc agcgcctcgg tgaccaccag gagccccatc gagccctcag
     1981 ccctggagcc ggcgtactac caaggtgtgt attccagacc cgtcctaaac acttcctacc
     2041 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
     2101 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
     2161 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2221 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2281 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2341 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2401 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2461 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2521 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     2581 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2641 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2701 gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac
     2761 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     2821 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     2881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     2941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3001 tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt
     3061 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     3121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3241 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3361 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     3421 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     3481 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3541 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3601 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3661 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3721 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3781 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3841 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3901 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3961 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4021 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     4081 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4141 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4201 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4261 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4321 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4381 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4441 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4501 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4561 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4621 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4681 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4741 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4801 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4861 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4921 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4981 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5041 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5101 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5161 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5221 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5281 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5341 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5401 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5461 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5521 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5581 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5641 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5701 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5761 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5821 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5881 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5941 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6001 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6061 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6121 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6181 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6241 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     6301 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     6361 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     6421 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     6481 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     6541 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6601 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6661 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     6721 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     6781 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     6841 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     6901 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     6961 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     7021 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     7081 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     7141 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     7201 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     7261 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     7321 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     7381 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     7441 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     7501 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     7561 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     7621 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     7681 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     7741 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     7801 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     7861 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     7921 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     7981 acctcccaac cccgagggga cccttgcgcc ttttccaagg cagccctggg tttgcgcagg
     8041 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     8101 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     8161 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     8221 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     8281 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     8341 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     8401 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     8461 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta ccaccggagc
     8521 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     8581 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     8641 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     8701 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc
     8761 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     8821 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     8881 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     8941 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     9001 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     9061 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     9121 cctgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     9241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     9301 acagcagaga tccactttgg cgccggctcg aggg
//
