LOCUS       sequence-124804-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF1-TF with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-132
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..155)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(435..455)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(456..479)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttaattaaca tatcgagatc gaaatcgtcc agagcatcgg agccgagcat gtccagatca
       61 aagtcatcca atgcgtcgct tcccaacata tccaggtcaa agtcatcgag ggcatcagaa
      121 cccagcatgt cgagatcgaa atcgtccagc gcgtcggcgc gcccggatcc tctcaggtgg
      181 gtttttaggt gtcttgacaa atgtggtttt tgactgaagt tgcgcatgca tattcggcat
      241 tggaatggct tctcgccggt gtgcgtacgt agatgtctaa ccaaatgttc ttgtctggag
      301 aaatttcgca tacagatccg acactgaaac ggtttttcac cggtatgagt acgggtatgt
      361 ctagtcaaag tatttttcat cgaaaagttc cgcatgcaaa tgcgacactg gaaggggcgc
      421 tccccgggtc tagagacctt gcgctttttc ttgggcttgt catcgtcgtc cttgtagtcc
      481 atgatatcac cggttgcttt gaattagcgg tggttttcac aacacctaaa aaagggttta
      541 aaagatacct ttgaaccgct aagaagcccg agaattagct ccgctcaaaa ctcaagggga
      601 caaattccaa aaatgacttc cagcgccagg ctggcctgac tagtctccac ccaccaaatg
      661 tgaacaaact ccaacgccat tacatcccct ccccccgccg cgactagccg tgctcaaaag
      721 cccgaggtga ctattgcggc cgataggacc acggggtcac aggaagcagc agccggtgag
      781 ggaccaggcc ctcttccttt gtgtgggtga ctcacccgcc cgctccaccg ggctgccgcg
      841 tc
//
