LOCUS       sequence-124811-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged
            with EGFP along with shRNA to knock down endogenous ch-TOG in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-ch-TOGKDP-GFP-shch-TOG
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccatggtggc gatggatcct ttgcgactgc tctttattct ctccagtctt tttttcaagt
       61 cgtctatgtt agctgtggag gaggaggagg tcacagttcc tgaagagtga gtctggttag
      121 aatccaggtc tgaatgctgg tgctgctccc gtgactcccg gagctgagac aacttggaat
      181 gtagcatgtc tgtggaagag gcgacagtag gaactgctgg tttggagagc aaagaggtca
      241 aaggaggtcg gtcatcttgc tttgtgttgt ccagaccaca tcgctgtcgg aggatcttta
      301 gcctttccaa gtagacagat ggccccactt cttccccatt tgtgttacct atggaggaca
      361 ctgtgcttgt gggcgtgggc acacatgtga cttccatctg aggggagatg cctgttgaag
      421 tggaaatacg acctttgccc tccctctcca tctcaatcac ccgaaggcct ctttcgacat
      481 agctctggaa gaactgtgag gaatttttca gaaatggttc aatgtcagca tctgagtatt
      541 tcttcttata ttcatataac tctgctagtc cctctttagt gttttcttta gagccaatct
      601 tcttaaaaat ctcagctaag aaatcattca ctttggcctt tgatgatttt tcatctattc
      661 gagatgctcc cttttctgtt tccttatcag acttgctccc agtctggtcc atactgtgct
      721 tcatcatccg gcagagatgg gcctccagct cagactcgtt t
//
