LOCUS       sequence-124812-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF4-TF with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-117
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..162)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(442..462)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(463..486)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttaacaatta attaacatat cgagatcgaa atcgtccaga gcatcggagc cgagcatgtc
       61 cagatcaaag tcatccaatg cgtcgcttcc caacatatcc aggtcaaagt catcgagggc
      121 atcagaaccc agcatgtcga gatcgaaatc gtccagcgcg tcggcgcgcc cggatcctct
      181 caggtgggtt tttaggtgat cacccaattt atcaattcta ctgaagttgc gcatgcatat
      241 tcggcattgg aatggcttct cgccggtgtg cgtacgtaga tgtctagtca aattagctct
      301 atcggagaaa tttcgcatac agatccgaca ctgaaacggt ttttcaccgg tatgagtacg
      361 ggtatgtcta gtcaaaacag cagcagtcga aaagttccgc atgcaaatgc gacactggaa
      421 ggggcgctcc ccgggtctag agaccttgcg ctttttcttg ggcttgtcat cgtcgtcctt
      481 gtagtccata gatctttgat atcaccggtt gctttgaatt agcggtggtt ttcacaacac
      541 ctaaaaaagg gtttaaaaga tacctttgaa ccgctaagaa gcccgagaat tagctccgct
      601 caaaactcaa ggggacaaat tccaaaaatg acttccagcg ccaggctggc ctgactagtc
      661 tccacccacc aaatgtgaac aaactccaac gccattacat cccctccccc cgccgcgact
      721 agccgtgctc aaaagcccga ggtgactatt gcggccgata ggaccacggg gtcacaggaa
      781 gcagcagccg gtgagggacc agg
//
