LOCUS       sequence-124814-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human ch-TOG with 
            L1939A and L1942A substitutions tagged with EGFP along with shRNA to
            knock down endogenous ch-TOG in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accatggtgg cgatggatcc tttgcgactg ctctttattc tctccagtct ttttttcaag
       61 tcgtctatgt tagctgtgga ggaggaggag gtcacagttc ctgaagagtg agtctggtta
      121 gaatccaggt ctgaatgctg gtgctgctcc cgtgactccc ggagctgaga caacttggaa
      181 tgtagcatgt ctgtggaaga ggcgacagta ggaactgctg gtttggagag caaagaggtc
      241 aaaggaggtc ggtcatcttg ctttgtgttg tccagaccac atcgctgtcg ggcgatcttt
      301 gccctttcca agtagacaga tggccccact tcttccccat ttgtgttacc tatggaggac
      361 actgtgcttg tgggcgtggg cacacatgtg acttccatct gaggggagat gcctgttgaa
      421 gtggaaatac gacctttgcc ctccctctcc atctcaatca cccgaaggcc tctttcgaca
      481 tagctctgga agaactgtga ggaatttttc agaaatggtt caatgtcagc atctgagtat
      541 ttcttcttat attcatataa ctctgctagt ccctctttag tgttttcttt agagccaatc
      601 ttcttaaaaa tctcagctaa gaaatcattc actttggcct ttgatgattt ttcatctatt
      661 cgagatgctc ccttttctgt ttccttatca gacttgctcc cagtctggtc catactgtgc
      721 ttcatcatcc ggcagagatg g
//
