LOCUS       sequence-124815-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF5-TF with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-127
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..161)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(441..461)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(462..485)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttacaattaa ttaacatatc gagatcgaaa tcgtccagag catcggagcc gagcatgtcc
       61 agatcaaagt catccaatgc gtcgcttccc aacatatcca ggtcaaagtc atcgagggca
      121 tcagaaccca gcatgtcgag atcgaaatcg tccagcgcgt cggcgcgccc ggatcctctc
      181 aggtgggttt ttaggtgtct attcaaagca tcttttctac tgaagttgcg catgcatatt
      241 cggcattgga atggcttctc gccggtgtgc gtacgtagat gagtagacaa attatccatt
      301 ctggagaaat ttcgcataca gatccgacac tgaaacggtt tttcaccggt atgagtacgg
      361 gtatgtctaa ccaatctttc acctttcgaa aagttccgca tgcaaatgcg acactggaag
      421 gggcgctccc cgggtctaga gaccttgcgc tttttcttgg gcttgtcatc gtcgtccttg
      481 tagtccatga tatcaccggt tgctttgaat tagcggtggt tttcacaaca cctaaaaaag
      541 ggtttaaaag atacctttga accgctaaga ancccgagaa ttagctccgc tcaaaactca
      601 aggggacaaa ttccaaaaat gacttccagc gccaggctgg cctgactagt ctccacccac
      661 caaatgtgaa caaactccaa cgccattaca tcccct
//
