LOCUS       sequence-124817-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     Sequence Label: pYLCRISPR/Cas9Pubi-B
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    33..207
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(214..765)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
       61 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
      121 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg
      181 aactactcac acattattat ggagaaactc gagtcaaatc tcggtgacgg gcaggaccgg
      241 acggggcggt accggcaggc tgaagtccag ctgccagaaa cccacgtcat gccagttccc
      301 gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg cctcgtgcat
      361 gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga agccctgtgc
      421 ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc gctggtggcg
      481 gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg
      541 gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc agggatagcg
      601 ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa
      661 gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc
      721 ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtag actcgagaga
      781 gatagatttg tagagagaga ctggtgatt
//
