LOCUS       sequence-124843-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF8-TF with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-131
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..166)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(446..466)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(467..490)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcttaaca attaattaac atatcgagat cgaaatcgtc cagagcatcg gagccgagca
       61 tgtccagatc aaagtcatcc aatgcgtcgc ttcccaacat atccaggtca aagtcatcga
      121 gggcatcaga acccagcatg tcgagatcga aatcgtccag cgcgtcggcg cgcccggatc
      181 ctctcaggtg ggtttttagg tgtctatcca aattaacagt ttgactgaag ttgcgcatgc
      241 atattcggca ttggaatggc ttctcgccgg tgtgcgtacg tagatgattt tccaaaactt
      301 ctcttctgga gaaatttcgc atacagatcc gacactgaaa cggtttttca ccggtatgag
      361 tacgggtatg tcttctcaaa acagccatag tcgaaaagtt ccgcatgcaa atgcgacact
      421 ggaaggggcg ctccccgggt ctagagacct tgcgcttttt cttgggcttg tcatcgtcgt
      481 ccttgtagtc catgatatca ccggttgctt tgaattagcg gtggttttca caacacctaa
      541 aaaagggttt aaaagatacc tttgaaccgc taagaagccc gagaattagc tccgctcaaa
      601 actcaagggg acaaattcca aaaatgactt ccagcgccag gctggcctga ctagtctcca
      661 cccaccaaat gtgaacaaac tccaacgcca ttacatcccc tccccccgcc gcgactagcc
      721 gtgctcaaaa gcccgaggtg actattgcgg ccgataggac cacggggtca caggaagcag
      781 cagccggtga gggaccaggc cctcttcctt tgtgtgggtg actcac
//
