LOCUS       sequence-124847-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9-TF with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-113
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..157)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(437..457)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(458..481)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aattaattaa catatcgaga tcgaaatcgt ccagagcatc ggagccgagc atgtccagat
       61 caaagtcatc caatgcgtcg cttcccaaca tatccaggtc aaagtcatcg agggcatcag
      121 aacccagcat gtcgagatcg aaatcgtcca gcgcgtcggc gcgcccggat cctctcaggt
      181 gggtttttag gtgtctttgc aaagaagtag attgactgaa gttgcgcatg catattcggc
      241 attggaatgg cttctcgccg gtgtgcgtac gtagatgtct agtcaaatta tgtctaacgg
      301 agaaatttcg catacagatc cgacactgaa acggtttttc accggtatga gtacgggtat
      361 gaactctcaa tttagtttta tccgaaaagt tccgcatgca aatgcgacac tggaaggggc
      421 gctccccggg tctagagacc ttgcgctttt tcttgggctt gtcatcgtcg tccttgtagt
      481 ccatagatct ttgatatcac cggttgcttt gaattagcgg tggttttcac aacacctaaa
      541 aaagggttta aaagatacct ttgaaccgct aagaagcccg agaattagct ccgctcaaaa
      601 ctcaagggga caaattccaa aaatgacttc cagcgccagg ctggcctgac tagtctccac
      661 ccaccaaatg tgaacaaact ccaacgccat tacatcccct ccccccgccg cgactagccg
      721 tgctcaaaag cccgaggtga ctattgcgg
//
