LOCUS       sequence-124848-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9-TF with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-113
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(96..806)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWQASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(374..393)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     643..661
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aaacgcagta caaagtgtta cccctctaga cctggaaaga ccaggcggag tttcaagttg
       61 cacgttttgt gtcattgggg aaacctgctc ctaggttact tgtacagctc gtccatgccg
      121 ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg
      181 tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc
      241 ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc
      301 ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc
      361 tgccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc
      421 agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc
      481 acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc
      541 ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac
      601 tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg
      661 gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg
      721 ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg
      781 ttatcctcct cgcccttgct caccatgtat actccggatt gctt
//
