LOCUS       sequence-124867-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(404..430)
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             complement(443..532)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(533..553)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gtcattaccc ggggtctttc tgacggaggt
       61 ggatcttggt gtgccgtgtc aggtggtggc tcagcgcgaa cttcctgccg cagatgtcac
      121 aggcgaaggg cttttctcct gtgtgggtgc ggatgtgccg tgtcaggctg ctacggatgc
      181 tgaagttcct catgcagatg cggcattgaa acggtttttg ccctgtgtga atgcggatgt
      241 gccgtgtcag gtgtcccttc tgactgaagc ggcggtcaca ggactcgaca ggacacgcgt
      301 atggcttctc accagtgtgc gtacgttggt gccgtgtcag gtcgtcgctc cgcgagaagg
      361 acttgccaca ttcaggacat ttatagggct ttccggattc gaaatccagt ttctttttct
      421 tagctgccgg agcggcggat ccagcgtaat ctggaacgtc ataaggatac gatcctgcat
      481 agtccgggac gtcataggga tagcccgcat agtcaggaac atcgtatggg tataccttcc
      541 tcttcttctt gggcccgccg gtgccgtctc caccgagctg agagaggtcg attcttgttt
      601 catagagccc cgtaattgac tgatgaatca gtgtggcgtc caggacctcc tttgtagagg
      661 tgtacttctt tctgtctatg gtggtgtcga agtacttgaa ggctgcaggc gcgcccaagt
      721 tggtcagagt aaacaagtgg ataatgtttt ctgcctgctc cc
//
