LOCUS       sequence-124868-         330 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3
FEATURES             Location/Qualifiers
     source          1..330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggagccct
       61 cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag
      121 atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc
      181 taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa
      241 aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa
      301 gtacccaacc atatatcatc tgaggaagaa
//
