LOCUS       sequence-124877-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OT-II TCR a chain cDNA in pCRII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Barnden MJ, Allison J, Heath WR, Carbone FR
  TITLE     Defective TCR expression in transgenic mice constructed using 
            cDNA-based alpha- and beta-chain genes under the control of 
            heterologous regulatory elements.
  JOURNAL   Immunol Cell Biol. 1998 Feb;76(1):34-40.
  PUBMED    9553774
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunol 
            Cell Biol."; date: "1998-02"; volume: "76(1)"; pages: "34-40"
COMMENT     Sequence Label: pCRII-1.1-1.10a
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     197..219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     211..228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     212..228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     235..254
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        235..253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctggcagtgt acggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
       61 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
      121 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      181 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
      241 gactcactat agggcgaatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat
      301 ggatatctgc agaattcggc ttggatccag gaatggacaa gattctgaca gcaacgtttt
      361 tactcctagg ccttcaccta gctggggtga atggccagca gcaggagaaa cgtgaccagc
      421 agcaggtgag acaaagtccc caatctctga cagtctggga aggagagacc gcaattctga
      481 actgcagtta tgaggacagc acttttaact acttcccatg gtaccagcag ttccctgggg
      541 aaggccctgc actcctgata tccatacgtt cagtgtccga taaaaaggaa gatggacgat
      601 tcacaatctt cttcaataaa agggagaaaa agctctcctt gcacatcaca gactctcagc
      661 ctggagactc agctacctac ttctgtgcag caaggggtaa cagaatcttc tttggtgatg
      721 ggacgcagct ggtggtgaag cccaacatcc agaacccaga acctgctgtg taccagttaa
      781 aagatcctcg gtctcaggac agcaccctct gcctgttcac cgactttgac tcc
//
