LOCUS       sequence-124894-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atacgactca ctatagttct agatatctcg aggcggccgc ttatccggat taagatcccg
       61 cgactcgatg ggaagttctc tcgggaatcc gccggttggt tcttttgatc aatgcgggag
      121 catgaggcag accctttttg actgcatcga gcgcgggtcg tccaccaaga catgccaacg
      181 ccaccagatg gtcattcgtt aacgcagcca acgcgggatc gggcctggac aattgggcta
      241 cgatggactc caaggctggc cgtcctccgt tattattagc gatagccacc acttggtccg
      301 gggtcaggcc atggtcctgg cacagcaccg gcaacagccg ctgcaccgtt tcgagcgctt
      361 gcttgccgcc attgttgctg gcgatagcca ccacttggtc cggggtcagg ccatggtcct
      421 ggcacagcac cggcaacagc cgctgcaccg tttcgagcgc ttgcttgccg ccaatgttgc
      481 tggcgatagc caccacttgg tccggggtca ggccatggtc ctggcacagc accggcaaca
      541 gccgctgcac cgtttcgagc gcttgcttgc cgccaatgtt gctggcgata gccaccactt
      601 ggtccggggt caggccatgg tcctggcaca gcaccggcaa cagccgctgc accgtttcga
      661 gcgcttgctt gccgccattg ttgctggcga tagccaccac ttggtccggg gtcaggccat
      721 ggtcctggca cagcaccggc aacagccgct gcaccgtttc gagcgcttgc
//
