LOCUS       sequence-124899-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF8/ZF7-TF with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-148
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..154)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(698..718)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(719..742)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 taattaacat atcgagatcg aaatcgtcca gagcatcgga gccgagcatg tccagatcaa
       61 agtcatccaa tgcgtcgctt cccaacatat ccaggtcaaa gtcatcgagg gcatcagaac
      121 ccagcatgtc gagatcgaaa tcgtccagcg cgtcggcgcg cccggatcct ctcaggtggg
      181 tttttaggtg tcttttcaaa gaagcaccaa cactgaagtt gcgcatgcat attcggcatt
      241 ggaatggctt ctcgccggtg tgcgtacgta gatgattagt caaatgtggt ctttgggaga
      301 aatttcgcat acagatccga cactgaaacg gtttttcacc ggtatgagta cgggtatgtc
      361 tatgcaaatg atcttttttc gaaaagttcc gcatgcaaat gcgacactgg aaggggcgct
      421 ccccgtcctt ctgtctcagg tgggttttta ggtgtctatc caaattaaca gtttgactga
      481 agttgcgcat gcatattcgg cattggaatg gcttctcgcc ggtgtgcgta cgtagatgat
      541 tttccaaaac ttctcttctg gagaaatttc gcatacagat ccgacactga aacggttttt
      601 caccggtatg agtacgggta tgtcttctca aaacagccat agtcgaaaag ttccgcatgc
      661 aaatgcgaca ctggaagggg cgctccccgg gtctagagac cttgcgcttt ttcttgggct
      721 tgtcatcgtc gtccttgtag tccatgatat caccggttgc tttgaattag cggtggtttt
      781 cacaacacct aaa
//
