LOCUS       sequence-124908-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF7-ZF8 Op 0gap -- GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-161
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(55..86)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    151..242
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(183..202)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    256..304
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggctt taccaacagt accggattgc caagctggaa
       61 gtcgagcttc cattatatac cctctagtgt ctaagcttgg accacttccc ctccagctgc
      121 tagcgagctc aggtaccggc cagttaggcc agagaaatgt tctggcacct gcacttgcac
      181 tggggacagc ctattttgct agtttgtttt gtttcgtttt gttttgatgg agagcgtatg
      241 ttagtactat cgattcacac aaaaaaccaa cacacagatg taatgaaaat aaagatattt
      301 tattgcggcc tgtccaatac ctcccgtacc ttaatattac ttacttatcc ttgagagacg
      361 tactagtaac cctgataaat gcttcaataa tattgaaaaa ggacgagtat gagcatccaa
      421 cattttcgtg tcgcactcat tcccttcttt gcggcatttt gcttgcctgt ttttgcacac
      481 cccgaaacgc tggtgaaagt aaaagatgct gaagatcaac tgggtgcaag agtgggctat
      541 atcgaactgg atctcaatag cggcaagatc cttgagtctt tccgccccga agaacgattc
      601 ccgatgatga gcactttcaa agtactgcta tgtggcgcgg tgttgtcccg tatagacgcc
      661 gggcaagagc agcttggtcg ccgtatacac tactcacaaa acgacttggt tgagtactcg
      721 ccggtcacgg aaaagcatc
//
