LOCUS       sequence-124910-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF7-ZF8 Op 1gap -- GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-163
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(55..86)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    152..243
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(184..203)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    257..305
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggctt taccaacagt accggattgc caagctggaa
       61 gtcgagcttc cattatatac cctctagtgt ctaagcttgg accacttcac cctccagctg
      121 ctagcgagct caggtaccgg ccagttaggc cagagaaatg ttctggcacc tgcacttgca
      181 ctggggacag cctattttgc tagtttgttt tgtttcgttt tgttttgatg gagagcgtat
      241 gttagtacta tcgattcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt
      301 ttattgcggc ctgtccaata cctcccgtac cttaatatta cttacttatc cttgagagac
      361 gtactagtaa ccctgataaa tgcttcaata atattgaaaa aggacgagta tgagcatcca
      421 acattttcgt gtcgcactca ttcccttctt tgcggcattt tgcttgcctg tttttgcaca
      481 ccccgaaacg ctggtgaaag taaaagatgc tgaagatcaa ctgggtgcaa gagtgggcta
      541 tatcgaactg gatctcaata gcggcaagat ccttgagtct ttccgccccg aagaacgatt
      601 cccgatgatg agcactttca aagtactgct atgtggcgcg gtgttgtccc gtatagacgc
      661 cgggcaagag cagcttggtc gccgtataca ctactcacaa aacgacttgg ttgagtactc
      721 gccggtcacg gaaaagcatc ttacggatgg catgacggta agagaattgt gtagtgctgc
      781 cattaccatg agcgacaaca ccgcggccaa cttacttctg acaacgatcg ga
//
