LOCUS       sequence-124916-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9 Op x4 -- GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-191
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(55..86)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    184..275
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(216..235)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    289..337
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggctt taccaacagt accggattgc caagctggaa
       61 gtcgagcttc cattatatac cctctagtgt ctaagcttct ccatctacat ttctccatct
      121 acatttctcc atctacattt ctccatctac agctagcgag ctcaggtacc ggccagttag
      181 gccagagaaa tgttctggca cctgcacttg cactggggac agcctatttt gctagtttgt
      241 tttgtttcgt tttgttttga tggagagcgt atgttagtac tatcgattca cacaaaaaac
      301 caacacacag atgtaatgaa aataaagata ttttattgcg gcctgtccaa tacctcccgt
      361 accttaatat tacttactta tccttgagag acgtactagt aaccctgata aatgcttcaa
      421 taatattgaa aaaggacgag tatgagcatc caacattttc gtgtcgcact cattcccttc
      481 tttgcggcat tttgcttgcc tgtttttgca caccccgaaa cgctggtgaa agtaaaagat
      541 gctgaagatc aactgggtgc aagagtgggc tatatcgaac tggatctcaa tagcggcaag
      601 atccttgagt ctttccgccc cgaagaacga ttcccgatga tgagcacttt caaagtactg
      661 ctatgtggcg cggtgttgtc ccgtatagac gccgggcaag agcagcttgg tcgccgtata
      721 cactactcac aaaacgactt ggttgagtac tcgccggtca cggaaaagca tcttacggat
      781 ggcatgacgg taagagaatt gtgtagtgct gccattacca tgagcgacaa caccgcggcc
      841 aacttacttc tgacaacgat cggaggccct aaggagctga ctgcatttct tcataatatg
      901 ggtgatcatg tgacccgg
//
