LOCUS       sequence-124918-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9 Op x6 -- GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-192
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(55..86)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    212..303
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(244..263)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    317..365
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggctt taccaacagt accggattgc caagctggaa
       61 gtcgagcttc cattatatac cctctagtgt ctaagcttct ccatctacat ttctccatct
      121 acatttctcc atctacattt ctccatctac atttctccat ctacatttct ccatctacag
      181 ctagcgagct caggtaccgg ccagttaggc cagagaaatg ttctggcacc tgcacttgca
      241 ctggggacag cctattttgc tagtttgttt tgtttcgttt tgttttgatg gagagcgtat
      301 gttagtacta tcgattcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt
      361 ttattgcggc ctgtccaata cctcccgtac cttaatatta cttacttatc cttgagagac
      421 gtactagtaa ccctgataaa tgcttcaata atattgaaaa aggacgagta tgagcatcca
      481 acattttcgt gtcgcactca ttcccttctt tgcggcattt tgcttgcctg tttttgcaca
      541 ccccgaaacg ctggtgaaag taaaagatgc tgaagatcaa ctgggtgcaa gagtgggcta
      601 tatcgaactg gatctcaata gcggcaagat ccttgagtct ttccgccccg aagaacgatt
      661 cccgatgatg agcactttca aagtactgct atgtggcgcg gtgttgtccc gtatagacgc
      721 cgggcaagag cagcttggtc gccgtataca ctactcacaa aacgacttgg ttgagtactc
      781 gccggtcacg gaaaagcatc
//
