LOCUS       sequence-124920-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9-TF-V1 with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-246
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..171)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(499..519)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(520..543)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagctagct taacaattaa ttaacatatc gagatcgaaa tcgtccagag catcggagcc
       61 gagcatgtcc agatcaaagt catccaatgc gtcgcttccc aacatatcca ggtcaaagtc
      121 atcgagggca tcagaaccca gcatgtcgag atcgaaatcg tccagcgcgt cggcgcgccc
      181 tccgctgccg ttgcctctct cgccgcagcc cacgtagatg attccgccgg atcctctcag
      241 gtgggttttt aggtgtcttt gcaaagaagt agattgactg aagttgcgca tgcatattcg
      301 gcattggaat ggcttctcgc cggtgtgcgt acgtagatgt ctagtcaaat tatgtctaac
      361 ggagaaattt cgcatacaga tccgacactg aaacggtttt tcaccggtat gagtacgggt
      421 atgaactctc aatttagttt tatccgaaaa gttccgcatg caaatgcgac actggaaggg
      481 gcgctccccg ggtctagaga ccttgcgctt tttcttgggc ttgtcatcgt cgtccttgta
      541 gtccatagat ctttgatatc accggttgct ttgaattagc ggtggttttc acaacaccta
      601 aaaaagggtt taaaagatac ctttgaaccg ctaagaagcc cgagaattag ctccgctcaa
      661 aactcaaggg gacaaattcc aaaaatgact tccagcgcca ggctggcctg actagtctcc
      721 acccaccaaa tgtgaacaaa ctccaacgcc attacatccc ctccccccgc cgcgactagc
      781 cgtgctcaaa agcccgaggt gactattgcg gccgatagga ccacggggtc acaggaagca
      841 gcagccggtg aggg
//
