LOCUS       sequence-124921-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9-TF-V1 with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-246
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(108..818)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWQASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(386..405)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     655..673
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gagcgtggag ccaaacgcag tacaaagtgt tacccctcta gacctggaaa gaccaggcgg
       61 agtttcaagt tgcacgtttt gtgtcattgg ggaaacctgc tcctaggtta cttgtacagc
      121 tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg
      181 tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc
      241 tgcacgggct tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc
      301 ttcagcttca gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc
      361 tcggaggagg cctgccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag
      421 ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg
      481 gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg
      541 gggaaggaca gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag
      601 ccgtacatga actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc
      661 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
      721 tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg
      781 atgatggcca tgttatcctc ctcgcccttg ctcaccatgt atactccgga ttgctttgaa
      841 ttagcggtgg c
//
