LOCUS       sequence-124923-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9-TF-V2 with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-247
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..168)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(496..516)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(517..540)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctagcttaa caattaatta acatatcgag atcgaaatcg tccagagcat cggagccgag
       61 catgtccaga tcaaagtcat ccaatgcgtc gcttcccaac atatccaggt caaagtcatc
      121 gagggcatca gaacccagca tgtcgagatc gaaatcgtcc agcgcgtcgg cgcgccctcc
      181 gctgcccttc tcggtcatgg tgcagccctt ctccagcact ccgccggatc ctctcaggtg
      241 ggtttttagg tgtctttgca aagaagtaga ttgactgaag ttgcgcatgc atattcggca
      301 ttggaatggc ttctcgccgg tgtgcgtacg tagatgtcta gtcaaattat gtctaacgga
      361 gaaatttcgc atacagatcc gacactgaaa cggtttttca ccggtatgag tacgggtatg
      421 aactctcaat ttagttttat ccgaaaagtt ccgcatgcaa atgcgacact ggaaggggcg
      481 ctccccgggt ctagagacct tgcgcttttt cttgggcttg tcatcgtcgt ccttgtagtc
      541 catagatctt tgatatcacc ggttgctttg aattagcggt ggttttcaca acacctaaaa
      601 aagggtttaa aagatacctt tgaaccgcta agaagcccga gaattagctc cgctcaaaac
      661 tcaaggggac aaattccaaa aatgacttcc agcgccaggc tggcctgact agtctccacc
      721 caccaaatgt gaacaaactc caacgccatt acatcccctc cccccgccgc gactagccgt
      781 gctcaaaagc ccgaggtgac tattgcggcc gataggacca cggggtcaca ggaagcagca
      841 gccggtgagg gaccaggccc tcttcctttg tgt
//
