LOCUS       sequence-124925-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9-TF-V3 with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-248
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..160)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(488..508)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(509..532)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aacaattaat taacatatcg agatcgaaat cgtccagagc atcggagccg agcatgtcca
       61 gatcaaagtc atccaatgcg tcgcttccca acatatccag gtcaaagtca tcgagggcat
      121 cagaacccag catgtcgaga tcgaaatcgt ccagcgcgtc ggcgcgccct ccgctgcccc
      181 acagcacgct gtcgcagccc agtctccagc ctccgccgga tcctctcagg tgggttttta
      241 ggtgtctttg caaagaagta gattgactga agttgcgcat gcatattcgg cattggaatg
      301 gcttctcgcc ggtgtgcgta cgtagatgtc tagtcaaatt atgtctaacg gagaaatttc
      361 gcatacagat ccgacactga aacggttttt caccggtatg agtacgggta tgaactctca
      421 atttagtttt atccgaaaag ttccgcatgc aaatgcgaca ctggaagggg cgctccccgg
      481 gtctagagac cttgcgcttt ttcttgggct tgtcatcgtc gtccttgtag tccatagatc
      541 tttgatatca ccggttgctt tgaattagcg gtggttttca caacacctaa aaaagggttt
      601 aaaagatacc tttgaaccgc taagaagccc gagaattagc tccgctcaaa actcaagggg
      661 acaaaattcc aaaaatgact tccagcgcca ggctggcctg actagtctcc acccaccaaa
      721 tgtg
//
