LOCUS       sequence-124930-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..36
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             100..126
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
ORIGIN
        1 agagccgcgc cggcagctaa gaaaaagaaa ctggatcaat tgggcgctag cggtagtccc
       61 gggtctggag gtagcggctc aagtggccgt acggcagctc ctgcggcaaa aaagaaaaag
      121 ttggactctg aattcggaag cggaagcgac aagaagtact ccattgggct cgatatcggt
      181 accaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc
      241 aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg agccctcctg
      301 ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat
      361 acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag
      421 gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag
      481 cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac
      541 ccaaccatat atcatctgag gaagaagctg gtagacagta ctgataaggc tgacttgcgg
      601 ttgatctatc tcgcgctggc gcacatgatc a
//
