LOCUS       sequence-124931-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  qacR 2 downstream of a Tet promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
  TITLE     Engineering Transcriptional Regulator Effector Specificity Using 
            Computational Design and In Vitro Rapid Prototyping: Developing a 
            Vanillin Sensor.
  JOURNAL   ACS Synth Biol. 2015 Aug 19.
  PUBMED    26262913
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Aug 19."
COMMENT     Sequence Label: pES003 (pTet-qacR 2)
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             163..174
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             755..772
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgcgccgag aggctttaca ctttatgctt ccggctggtc tcagcatcca cgcattataa
       61 cgtcgtatta gctgcctttc gtcttcaata attcttgaca tccctatcag tgatagggat
      121 acttcgtgca atttttatat caccgccagg ggtacaacaa gcaaagagga gaaatactag
      181 catgaacttg aaagataaga ttttgggtgt tgctaaagag ctgtttatca aaaacggtta
      241 caatgctact accactggtg agattgtgaa attgtctgaa tcttctaaag ggaatctata
      301 ctatcatttc aaaacgaagg aaaatttgtt cttggaaatc ttgaacattc aactgagtaa
      361 atatcaagaa caatggaaaa aggagcagat taaagctaaa actaatagag aaaagtttta
      421 tctgtataat gagttgtctt tgactacaca atattactat ccactacaga acgcacagat
      481 cgagttctac actgaatact ataagactaa ctccatcaat gaaaaacaga acaaatacca
      541 aaataagtat atcgatgcat atcacgttat tttcaaagaa ggtaatctta acggtgagtg
      601 gagtataaat gatgttaatg ccgtttctaa aattgctgct atggctgttt taggtattgt
      661 tatgttcaca catgagcaaa acatcaacga aagaattaag ttgatgaaca agttctctca
      721 gatctttttg aatggtctta gtaaaggttc tggtcaccat catcaccacc attaatgaac
//
