LOCUS       sequence-124932-         322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALE for mouse major satellite with FLAG tag under CAG 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Ishiuchi T, Enriquez-Gasca R, Mizutani E, Boskovic A, 
            Ziegler-Birling C, Rodriguez-Terrones D, Wakayama T, Vaquerizas JM, 
            Torres-Padilla ME
  TITLE     Early embryonic-like cells are induced by downregulating 
            replication-dependent chromatin assembly.
  JOURNAL   Nat Struct Mol Biol. 2015 Aug 3. doi: 10.1038/nsmb.3066.
  PUBMED    26237512
REFERENCE   2  (bases 1 to 322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2015 Aug 3. doi: 10.1038/nsmb.3066."
COMMENT     Sequence Label: pTAL-MajSat-FLAG-NLS-MCS
FEATURES             Location/Qualifiers
     source          1..322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             115..135
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat tggcgaacgc
       61 acgtcccatc gcgttgcggg atccatggac tacaaggacg acgatgacaa gagccccaag
      121 aagaagagaa aggtggaggc cagcgaattc gatatcggcg gccgcgtcga ctgataattc
      181 tgtacatatg ggcaccaaag aacctgtaaa cgttatcttt ttaaattgaa tgtgcacaaa
      241 taaaagtttg gaaaagaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      301 aaaaaaaaaa aaaaaaaaaa aa
//
