LOCUS       sequence-124934-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9-TF-V2 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-273
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             128..148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             476..625
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             644..661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
       61 atcattttgg caaagaattg attaattcga gcgaacgcgt atggactaca aggacgacga
      121 tgacaagccc aagaaaaagc gcaaggtctc tagacccggg gagcgcccct tccagtgtcg
      181 catttgcatg cggaactttt cggataaaac taaattgaga gttcataccc gtactcatac
      241 cggtgaaaaa ccgtttcagt gtcggatctg tatgcgaaat ttctccgtta gacataattt
      301 gactagacat ctacgtacgc acaccggcga gaagccattc caatgccgaa tatgcatgcg
      361 caacttcagt caatctactt ctttgcaaag acacctaaaa acccacctga gaggatccgg
      421 cggagtgctg gagaagggct gcaccatgac cgagaagggc agcggagggc gcgccgacgc
      481 gctggacgat ttcgatctcg acatgctggg ttctgatgcc ctcgatgact ttgacctgga
      541 tatgttggga agcgacgcat tggatgactt tgatctggac atgctcggct ccgatgctct
      601 ggacgatttc gatctcgata tgttaattaa ttgtggatct ggccaccatc accatcacca
      661 ttaagcggcc gcactcctca ggtgcaggct gcctatca
//
