LOCUS       sequence-124935-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9-TF-V2 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-273
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    128..149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(164..169,170..187,188..194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    202..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     207..229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     226..242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     226..242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     585..604
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    603..658
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     704..723
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 cgcctctccc gcgcgttggc cgattcatta atgcagcgga tctctcgagt taatttcgct
       61 taatttcgcg gatccgctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      241 accatgatta cgccaagctt gggctgcagg tcgagggatc tccataagag aagagggaca
      301 gctatgactg ggagtagtca ggagaggagg aaaaatctgg ctagtaaaac atgtaaggaa
      361 aattttaggg atgttaaaga aaaaaataac acaaaacaaa atataaaaaa aatctaacct
      421 caagtcaagg cttttctatg gaataaggaa tggacagcag ggggctgttt catatactga
      481 tgacctcttt atagccaacc tttgttcatg gcagccagca tatgggcata tgttgccaaa
      541 ctctaaacca aatactcatt ctgatgtttt aaatgatttg ccctcccata tgtccttccg
      601 agtgagagac acaaaaaatt ccaacacact attgcaatga aaataaattt cctttattag
      661 ccagaagtca gatgctcaag gggcttcatg atgtccccat aatttttggc agagggaaaa
      721 agatctcagt ggtatttgtg agccagggca ttggccacac cagccaccac cttctgatag
      781 gcagcctgca cc
//
