LOCUS       sequence-124937-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V1 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-276
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             137..157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
       61 tgctgtctca tcattttggc aaagaattga ttaattcgag cgaacgcgta tggactacaa
      121 ggacgacgat gacaagccca agaaaaagcg caaggtctct agacccgggg agcgcccctt
      181 ccagtgtcgc atttgcatgc ggaacttttc ggataaaact aaattgagag ttcatacccg
      241 tactcatacc ggtgaaaaac cgtttcagtg tcggatctgt atgcgaaatt tctccgttag
      301 acataatttg actagacatc tacgtacgca caccggcgag aagccattcc aatgccgaat
      361 atgcatgcgc aacttcagtc aatctacttc tttgcaaaga cacctaaaaa cccacctgag
      421 aggatccggc ggaatcatct acgtgggctg ctttgccaag ggtaccaatg ttttaatggc
      481 ggatgggtct attgaatgta ttgaaaacat tgaggttggt aataaggtca tgggtaaaga
      541 tggcagacct cgtgaggtaa ttaaattgcc cagaggaaga gaaactatgt acagcgtcgt
      601 gcagaaaagt cagcacagag cccacaaaag tgactcaagt cgtgaagtgc cagaattact
      661 caagtttacg tgtaatgcga cccatgagtt ggttgtt
//
