LOCUS       sequence-124939-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V1 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-276
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    130..151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(166..171,172..189,190..196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    204..220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     209..231
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     228..244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     228..244
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     587..606
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    605..660
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     706..725
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 cacgcctctc ccgcgcgttg gccgattcat taatgcagcg gatctctcga gttaatttcg
       61 cttaatttcg cggatccgct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      121 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      181 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      241 tgaccatgat tacgccaagc ttgggctgca ggtcgaggga tctccataag agaagaggga
      301 cagctatgac tgggagtagt caggagagga ggaaaaatct ggctagtaaa acatgtaagg
      361 aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac
      421 ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact
      481 gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca
      541 aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc
      601 cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt
      661 agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa
      721 aaagatctca gtggtatttg tgagccaggg cattggccac accagccacc accttctgat
      781 aggcagcctg cacctgagga
//
