LOCUS       sequence-124940-         505 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V1m with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-277
FEATURES             Location/Qualifiers
     source          1..505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             123..143
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gactcatcat
       61 tttggcaaag aattgattaa ttcgagcgaa cgcgtatgga ctacaaggac gacgatgaca
      121 agcccaagaa aaagcgcaag gtctctagac ccggggagcg ccccttccag tgtcgcattt
      181 gcatgcggaa cttttcggat aaaactaaat tgagagttca tacccgtact cataccggtg
      241 aaaaaccgtt tcagtgtcgg atctgtatgc gaaatttctc cgttagacat aatttgacta
      301 gacatctacg tacgcacacc ggcgagaagc cattccaatg ccgaatatgc atgcgcaact
      361 tcagtcaatc tacttctttg caaagacacc taaaaaccca cctgagagga tccggcggaa
      421 tcatctacgt gggcgccttt gccaagggta ccaatgtttt aatggcggat gggtctattg
      481 aatgtattga aaacattgag gttgg
//
