LOCUS       sequence-124941-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V1m with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-277
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    118..139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(154..159,160..177,178..184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    192..208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     197..219
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     216..232
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     216..232
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     575..594
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    593..648
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     694..713
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(803..820)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgga tctctcgagt taatttcgct taatttcgcg
       61 gatccgctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      121 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      181 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      241 cgccaagctt gggctgcagg tcgagggatc tccataagag aagagggaca gctatgactg
      301 ggagtagtca ggagaggagg aaaaatctgg ctagtaaaac atgtaaggaa aattttaggg
      361 atgttaaaga aaaaaataac acaaaacaaa atataaaaaa aatctaacct caagtcaagg
      421 cttttctatg gaataaggaa tggacagcag ggggctgttt catatactga tgacctcttt
      481 atagccaacc tttgttcatg gcagccagca tatgggcata tgttgccaaa ctctaaacca
      541 aatactcatt ctgatgtttt aaatgatttg ccctcccata tgtccttccg agtgagagac
      601 acaaaaaatt ccaacacact attgcaatga aaataaattt cctttattag ccagaagtca
      661 gatgctcaag gggcttcatg atgtccccat aatttttggc agagggaaaa agatctcagt
      721 ggtatttgtg agccagggca ttggccacac cagccaccac cttctgatag gcagcctgca
      781 cctgaggagt gcggcccgct taatggtgat ggtgatggtg g
//
